Squamous cell carcinoma of the lung histological features, lung squamous cell carcinoma of different histological differentiation characteristics:
well-differentiated squamous cell carcinoma (well-differentiated): rare, its features are: cancer nest was layered structures, like basal cell carcinoma of pericytes, cells in the central area cell nests may spike cells, cancer cells with a wide angle of some tumor nests forming keratin pearls, intercellular bridges clearly visible.
moderately differentiated squamous cell carcinoma (moderately differentiated): more see, about 26%. Main features are: cancer nest clearly stratified, keratinized cells is easy to see, showing keratin pearls and intercellular bridges.
poorly differentiated squamous cell carcinoma (poorly differentiated): the most common, accounting for about 63% of lung squamous cell carcinoma. Its characteristics are: cancer nest unclear hierarchical structure is easier to see the cancer tissue necrosis, but the bridge between cancer and cell keratosis rare.
Squamous cell carcinoma of the lung histological features (2) squamous cell carcinoma of the lung in the differential diagnosis:
poorly differentiated squamous cell carcinoma and large cell carcinoma of the main distinguishing points: a. poorly differentiated squamous cell carcinoma of the nest was layered structures, and large cell carcinoma of the cancer nest filled, non-hierarchical structure; b. poorly differentiated squamous atypia low in large cell carcinoma, and large cell carcinoma cell necrosis is more common.
poorly differentiated squamous cell carcinoma and adenosquamous carcinoma of the identification of: lung squamous cell carcinoma of the HE section, and sometimes see a small number of cells within the mucus formation, but the lung squamous cell carcinoma of the clear characteristics of squamous differentiation, and cancer cells containing liquid Education a minority, it can not be diagnosed as adenosquamous carcinoma. Generally speaking, the diagnosis of mixed carcinoma of the mixed composition is not less than 10% of the whole.
Squamous cell carcinoma of the lung histological features (3) squamous cell carcinoma of the lung by electron microscopy and immunohistochemical characteristics:
electron microscopic features: the same or organs in other parts of the ultrastructure of squamous cell carcinoma occurred in pulmonary squamous cell carcinoma of the ultrastructure of the main features are: the cytoplasm appears tension fiber (tonofibril) or tension, actin (tonofilament) . And the higher degree of differentiation, the more extensive the tension of the original fiber bundle; cancer occurs between the different levels of development of desmosomes (desmosome) and connections.
immunohistochemistry: in poorly differentiated squamous cell carcinoma on the diagnosis and identification of some significance. Squamous cell carcinoma of cytokeratins (Cytokeratin, CK) expression is diffuse positive. The higher differentiation, the more positive cells, the stronger the color. Positive rate was 100%. For epithelial membrane antigen (EMA) expression was also found. However, fewer positive cells, better color intensity. Of carcinoembryonic antigen (CEA) were positive for only 45.5% of cases.
Neuroendocrine antibodies against neuron-specific enolase (NSE), network prime grain (chromogranin), synaptic pro (synaptoPhysi the other 52.9% -81% of the cases also were positive. Squamous cell carcinoma of the lung, 50% -75% P53 protein was overexpressed; 15% Rb protein deficiencies; 80% of lung squamous cell carcinoma, epidermal growth factor receptor was overexpressed.