Variant of large cell carcinoma – large cell carcinoma with rhabdoid phenotype: the type of cancer cells was eosinophilic intracytoplasmic inclusion bodies with large cell carcinoma, a rare case.
the naked eye: the tumor border is unclear. Section gray-brown, often accompanied by focal hemorrhage and necrosis.
microscope: cell nucleus large, vacuolization, prominent nucleoli. Part of the cancer cell nucleus deviation. Diffuse distribution of cells, clear state. Morphological features with diagnostic significance is the large cell carcinoma of the part or most (at least 10%) cancer cells rich in cytoplasm, eosinophilic, and large round education strong eosinophilic inclusion body.
variant of large cell carcinoma – large cell carcinoma with rhabdoid phenotype electron microscopy: cell cytoplasm is rich in glycogen particles, while the light microscope, the strong eosinophilic inclusion bodies to intermediate filaments in the electron microscope. Visible cell processes of individual cases of cancer and cause the core particles land, namely neuroendocrine differentiation.
Special staining: a rhabdoid phenotype in tumor cells. Without amylase treatment, positive staining; after amylase treatment, were negative. Confirmed that contain sugar, but the cytoplasm of eosinophilic inclusion bodies PAS staining was negative.
immunohistochemistry: a result of cell cytoplasm containing abundant intermediate filaments, gas immunohistochemical staining, cells could simultaneously express vimentin, EMA and cytokeratin, but myogenic standard, antibodies [such as actin (actin) , myoglobin (myoglobin) and HHF-35, etc.]; the negative. If the tumor neuroendocrine differentiation occurs, can be presented NSE, chromogra11111 and synaptophysin positive.
variant of large cell carcinoma – large cell carcinoma with rhabdoid phenotype differential diagnosis
a. Carcinosarcoma: The difference is that the latter two components varied, the composition of cancer, including squamous cell carcinoma, sarcoma of the ingredients are mostly two or more, including fiber, nerve, muscle, bone, bone, animal husbandry. If the mother striated muscle cells, myoglobin immunohistochemistry (myogfobin), medium protein (desmin) was positive.
b. malignant melanoma: malignant melanoma cells can be seen with varying amounts of black particles, if necessary, immunohistochemistry, malignant melanoma cells S-100 protein HIIlMB-45-positive, these are associated with large cell carcinoma with rhabdoid table type are different.
The large cell carcinoma with rhabdoid phenotype and the identification of other tumors, according to Zheng and immunohistochemical tissue staining, is not difficult to identify.
prognosis: the strong invasion and poor prognosis.